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KMID : 1007519990080020128
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Food Science and Biotechnology
1999 Volume.8 No. 2 p.128 ~ p.133
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Functional Properties of Exopolysaccharide Produced from Zoogloea ramigera 115SLR by Genetic Modification
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Kim Chan-Shick
Lee Sam-Pil
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Abstract
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The gene involved in exopolysaccharide biosynthesis of Zoogloea ramigera 115SLR was manipulated using a gene disruption technique. An internal DNA fragment of gene encoding pyruvyltransferase was subcloned into a suicide vector, pUCD4121. Recombinant plasmids pUCD4345K and pUCD4198X were constructed and transformed into Z. ramigera 115SLR by electroporation. Mutant strains were classified into three groups, according to their pyruvate contents and physical properties of exopolysaccharide (zooglan). Group Ⅰ strains produced a less sticky and less viscous zooglan than did the wild-type Z. ramigera 115SLR strain. Group Ⅱ strains produced a sticky zooglan (100-200 ㎎/dL) with high pyruvate content. On the other hand, Group Ⅲ strains produced less zooglan (2-6 ㎎/dL) with lower pyruvate content (2%) and viscosity. Zooglans produced by various mutant strains showed the different functional properties in viscosity, hydrodynamic volume, calcofluor-binding and heavy metal-binding affinities.
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